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Here 1.5 10 5 CD8 + T cells were plated in Seahorse XF cell culture plates coated with poly-d-lysine (Sigma-Aldrich, P7280) and pre-incubated in appropriate medium at 37 C and in the absence of CO 2 for 1 h prior to treatments

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All GHRH tests were begun between the hours of 07:00 and 09:00

Categorical variables were compared using the Pearson 2 test, and continuous variables were compared using an independent-sample t test

Supraphysiological doses of intravenous PYY3-36 cause nausea, but no additional reduction in food intake