FBS was obtained from TransGen Biotech (Beijing, China)
Buffy coat (middle layer): This thin layer contains most of the white blood cells and a high concentration of platelets
It is stable, cost-effective, and the body converts it to its active forms efficiently
Lipedema causes symmetrical, disproportionate fat accumulation primarily in the legs and arms, predominantly affecting women
Informed consent was obtained from all individual participants involved in the study
Furthermore, this trend toward increased alpha diversity in the obese groups placed on an LD that had consequently experienced weight loss aligns with clinical observations, where low gene richness has been associated with increased adiposity, insulin resistance, and dyslipidemia 70 while increased gene richness has been observed following dietary intervention in obese and overweight individuals 71