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We believe that an enhanced understanding of [] the structural changes within intervertebral disc tissue and inflammatory responses will help identify novel therapeutic targets and enable effective treatment. Within the spine there are numerous chemical messengers released by neurons called neurotransmitters that carry signals and information between cells

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Columns were brought to room temperature and eluted 5 times with 100 L of elution buffer (1.5 M NaCl in lysis buffer) by centrifugation at 100 g for 1 min

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