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JH: Data curation, Investigation, Methodology, Validation, Writing review and editing

TB-500 studies examine epicardial progenitor cell mobilization and neovascularization processes

When preparing for an experiment, the vial should be allowed to equilibrate to room temperature before opening to prevent condensation, which can degrade the lyophilized powder

Defining a PVN Glu DMV Ach small intestine circuit To identify the cellular and/or molecular mechanism(s) mediating the observed suppressive effect of morphine on small intestine-projecting DMV Ach neuronal activity, we conducted retrograde monosynaptic tracing by infusing an AAV2/Retro-hSyn-Cre virus into the small intestine, along with a modified rabies virus (EnvA-pseudotyped RV-G-EGFP) and Cre-dependent helper viruses (AAV-Ef1-DIO-TVA-mCherry and AAV-Ef1-DIO-RVG) into the DMV of C57 mice (Fig

iii) complete DMEM containing HC at various concentrations (i.e., 1, 2, 4, 8, 10, 20 and 50 M) all containing 0.1% DMSO (as in all experiments described below)