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Evaluation of the brain tissue using immunohistochemistry and immunofluorescence showed an increase in both GFAP and Iba-1 positive cells in wild type mice treated with MPTP

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After the lysis of the cells with RIPA lysis buffer, the cellular protein levels were measured using a bicinchoninic acid assay (Beyotime)

Federal Food and Drug Administration (FDA) or the European Medicines Agency (EMA) [26]