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At 2 h post treatment, mice were injected intratracheally with either vehicle control (ethanol) or 25HC (5 mg/kg)

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[4] Studies evaluating the effect of peptide exposure in cell cultures have noted that Epithalon appears to directly interact with promoter regions of certain genes such as Tram1, IL-2, CD5, and MMP2 and may also enhance their expression

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Slowly add more nights until you reach your goal frequency once your skin feels comfortable