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Int J Mol Sci, 2021

Media was removed 4 h later and cells were lysed with 40 l TNT lysis buffer (20 mM Tris, pH 8, 200 mM NaCl, 1% triton-100)

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[DOI] [PubMed] [Google Scholar] 21.Colombo B., Saraceno L., Comi G

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Enzymatic Reaction Conditions The substrate solution used for the NsPCS reaction was prepared as follows: 28.7 mg of tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Tokyo Chemical Industry) and 3.0733 g of GSH (Nacalai Tesque) were weighed in a 100 mL volumetric flask, 20 mL of 1000 mM potassium phosphate buffer (pH 8.0) was added, and the solution was gently shaken