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Objective physiological data gathered from skin biopsies in this same study showed that after approximately six months of targeted supplementation with L-methylfolate, methylcobalamin, and pyridoxal 5'-phosphate, 73% of the patients demonstrated a measurable increase in calf epidermal nerve fiber density

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Individuals who benefit most from B12 shots for diagnosed deficiency are patients with conditions that impair gastrointestinal absorption, such as pernicious anemia, tropical sprue, or pancreatic insufficiency