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Dissected cochlear epithelia are transferred to permeable membrane inserts (Millicell organotypic culture plate inserts, Millipore) which are placed into 1 mL of culture media (Dulbecco's modified Eagle's medium (high glucose, Glutamax, 25 mM HEPES) with 10% fetal bovine serum, 1% N2 supplement and 10 units/ml penicillin) in a 6-well plate

This rationale supports a patient-selection hypothesis wherein KRAS-mutant tumors with high FSP1 expression are uniquely vulnerable to the combination of an FSP1 inhibitor and a ferroptosis inducer

Kim, J., Eygeris, Y., Gupta, M

Their storage requirements follow the general 21-to-28-day guideline when reconstituted, with sermorelin on the shorter end due to its somewhat more sensitive sequence