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By educating patients about benefits, honestly disclosing risks, screening for contraindications, and documenting everything clearly, you build trust, ensure safety, and create a defensible record if questions ever arise

Measurement of TMA, d 9 -TMA, TMAO, d 9 -TMAO, BB, d 9 -BB, carnitine, d 9 -carnitine, and choline levels in human and culture medium by LC-MS For sample preparation, 1 L of plasma, urine, or culture medium was mixed with 199 L of isotopically labeled internal standards (d 3 -carnitine, d 9 -TMAO, and 13 C 3 -TMAO) in 0.1% formic acid acetonitrile solution

The samples were treated with tris (2-carboxyethyl) phosphine (10 L, 55 mM in 50 mM TEAB, 30 min, 60 C) followed by treatment with chloroacetamide (10 L, 120 mM in 50 mM TEAB, 30 min, 25 C in the dark)

HEK293 cells were seeded in 6 well tissue culture plates and grown to 95% confluency in DMEM containing 10% FBS

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But NAC supplementation has further use as it has shown to slow down platelet aggregation