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Ru release of NP4 To examine the release profile of the nanoparticles, NP4 (Ru concentration: 800 M, 3 mL) was placed in a dialysis bag with a molecular weight cutoff of 3500 Da

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Ethics declarations Ethics approval and consent to participate The study was approved by the ethical review board of the Animal Ethics Committee of Central South University (CSU-2022-0116)

All values were converted to cAMP concentration using cAMP standard curve performed parallel and data were subsequently normalized to the response of 100M forskolin in each cell line, and then normalized to the WT for each peptide agonist

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