Moreover, it is very rare that any forms of vitamin B12 are found in plant-based foods, making a dietary supply of B12 difficult for vegans to obtain (see here: Vitamin B12 for Vegetarians and Vegans)
Half nuclei were bleached to reach 50% fluorescence drop at 100% laser power (0,45 mW)
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The PVDF membranes were incubated with the appropriate primary antibodies overnight at 4 C with gentle shaking, and then incubated with a goat anti-mouse/rabbit IgG horseradish peroxidase-conjugated antibody
