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Solvent Residues : Peptides synthesized via solid-phase methods may retain trace solvents like trifluoroacetic acid (TFA)

Your data becomes a chaotic mess, completely divorced from the scientific method

When the bac water is sub-spec, the math still produces a number but the resulting solution doesn't behave as expected

Lyophilised shelf life at -20C is significantly longer

After a few weeks I notice the difference

NNMT (nicotinamide N-methyltransferase) is a cytosolic enzyme that catalyzes a specific methylation reaction: it transfers a methyl group from S-adenosylmethionine (SAM) to nicotinamide, producing two products 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH)