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Furthermore, it was reported that the combination of mTORC1 inhibitors with IKE exhibits synergistic tumor suppression in lung cancer models (109)

Subsequently, 0.4 mL of a PI staining/lysis solution (0.5% NP-40, 0.5 mM ethylenediaminetetraacetic acid [EDTA]) and 50 L of RNase A (10 mg/mL in TrisEDTA buffer, pH 8.0) solution were added

To view a copy of this licence, visit About this article Cite this article Hwang, JH., Lee, HY., Chung, K.B

Measurement of the activity of senescence-associated -galactosidase (SA--gal) The activity of SA--gal in rats and patients liver tissues sections and cultured primary rat hepatocytes was determined using 5-bromo-4-chloro-3-indolyl P3-d-galactoside (X-gal), according to the manufacturer instruction (Senescence-associated -Galactosidase Staining Kit, Beyotime, C0602)

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