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Our facility operates under cGMP, ISO22000, ISO9001, FSSC22000, HALAL, KOSHER, BRC, and ORGANIC certifications, providing documented assurance that every aspect of production meets international quality management standards
Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C
In addition to MTT assays, we also performed cell counting with a Countess Automated Cell Counter (Applied Biosystems) in experiments where we treated primary melanoma cell lines WM115 and WM1862 with the EPAC inhibitor ESI-09 and transfected with EPAC siRNA
Use a gentle, pH-balanced cleanser that doesn't strip the skin or leave a residue
Flick the syringe to get any air bubbles to the top and gently push them out
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