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Mitochondria, endoplasmic reticulum (ER) and lysosomes can independently sense ROS levels and regulate local ROS microenvironment stability [140]

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The supernatant was applied to an Ni-nitroloacetic acid affinity column (Qiagen, Hilden, Germany) pre-equilibrated with lysis buffer and then washed with wash buffer comprising 50 mM sodium phosphate, 300 mM NaCl, and 20 mM imidazole (pH 8.0)

doi: 10.1016/j.yrtph.2006.05.006 45 HoleekM