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For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)
In addition to semaglutide, many patients were receiving other HS treatments, including adalimumab, infliximab, brodalumab, metformin, and spironolactone, reflecting the complex, multimodal management typical of HS [71]