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A digital camera (30 frames per second) mounted directly above the center of the maze captured each trial

At varied time points (0, 2, 6, 24, 48, 72, and 96 h), an aliquot solution (1 mL) was extracted from the mixture and centrifugated

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Rapid progression also prevents accurate assessment of what concentration actually delivers results

Average intensity values were plotted against distance in microns along the gonadal axis (x-axis, top), which were converted to the conventional metric of germ cell diameters from distal end (x-axis, bottom) (see Methods)

References 1 AASLD Practice Guidance on Noninvasive Assessment of Hepatic Fibrosis, Hepatology, 2024