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Author contributions CX conceived the research plans and supervised the experiments

Participants were largely positive about their trial experience, and perceived tirzepatide to be effective both for weight loss and subsequent improvements in aspects of daily life such as physical functioning, emotional well-being, and social life and relationships, which are frequently negatively impacted by living with obesity or overweight

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However, some of the widely used affinity tags, such as the His-tag, exhibit several drawbacks and limitations, since they can increase the risk of distorting the natural conformation of the target protein or necessitate stringent elution and wash conditions affecting the yield of the target protein