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The hunger strikes back: an epigenetic memory for autophagy

Emerging gene-editing technologies, particularly CRISPR/Cas9, now allow for the precise introduction of human disease-associated alleles or even the replacement of zebrafish genes with their human counterparts, creating humanized models that promise far greater fidelity in mimicking human pathology and pharmacological response (Haberlein et al., 2022

Unfortunately, glutathione can leak from microsomes, altering the [GSH]:[GSSG] ratio during their preparation, which would affect the values

A Vitamin Boost Might Be Just What You Need

For example, in the active reducing environment of the cytoplasm, it is difficult to form intramolecular and intermolecular protein disulfides when the GSH:GSSG ratio exceeds 100:1

When indicated, cells were exposed to menadione sodium bisulfite or N -acetyl-l-cysteine (NAC