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Briefly, FLS were transferred to 96-well plates (10,000/well) for 24 h and then incubated in various concentrations of l-carnitine (2.5, 5, 10, and 100 mM) or DMSO alone for 24 h

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When you first take it out of the fridge, note that date on the pen or vial, or keep a note on your calendar to keep track of how many days it's been unrefrigerated

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Fatty acid transport Long-chain fatty acids (the vast majority of fats stored in the body) cannot freely cross the mitochondrial membrane