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You wouldn't start a complex experiment without calibrating your instruments, and this is no different

Analytes (thermostated at 4 C) were separated through the following gradient of solvent B (minutes/%B): (0/3), (3.5/3), (15.5/75), (17.5/75) at a flow rate of 0.25 mL/min

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used MD simulations to identify residues near the active site that would shorten the distance between the phosphorus atom in PC and the catalytic H436, and the distance between the OH group of serine and H436, leading to more efficient synthesis of PS