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The LC column was a Millipore ZIC-pHILIC (2.1 150 mm, 5 m) coupled to a Dionex Ultimate 3000 system and the column oven temperature was set to 25 C for the gradient elution

Mitochondrial impairment may increase cellular NAD(P)H: Resazurin oxidoreductase activity, perturbing the NAD(P)H-based viability assays

One-way analysis of variance (ANOVA) was used to test continuous variables between multiple groups, and the least significant difference (LSD) was used for post hoc multiple comparisons

While clinical studies using NAD-boosters, glutathione or NAC in different diseases are increasing in number, their implementation as trial end-points and in clinical use is hampered by the lack of knowledge of their ranges in healthy population, especially in the blood, an accessible sample source

Separation was carried out using a non-isocratic method (20% - 50% methanol over 30 min) at 37C and a flow rate of 0.3 mL/min

Storage : Store at the coldest possible temperature (-80C) as a lyophilized powder