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In each generation, three libraries were generated at different levels of mutagenesis with the following primers: forward, 5- GGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGCTAGCCATATG-3

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10.1371/journal.pone.0165267 [DOI] [PMC free article] [PubMed] [Google Scholar] Sharma A