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A study from Norway found the 2.5th to 97.5th percentile among people aged 71 to 74 to be 100 to 360 nmol/l (Vogiatzoglou, 2009)

An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

10.1016/j.cell.2017.02.031 Cell 5 BorodkinaA

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While nuclear factor erythroid 2-related factor 2 (NRF2) and CCAAT/Enhancer Binding Protein (CEBP) also contribute to a minor increase in CHAC1 transcription, their roles are less pronounced compared to ATF4 ( FIGURE 4 ATF4 and ATF3 regulate CHAC1 expression by binding to specific sites within its promoter, the ATF/cAMP response element (CRE) at position 267 and the ATF/CRE modifier (ACM) motif at 248