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Insulin-like growth factor long arginine 3 (IGF-1 LR3), is a widely used synthetic analog of IGF-1 with an arginine replacing glutamic acid at position 3 (arginine 3, R3), and an additional 13 amino acids at its N-terminus (long, L)

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To assess melittins hemolytic activity in the presence of its binders, 10 L of melittin (1.2 M final) was added to wells of a 96-well plate, followed by serial dilution of protein (0.05 M to 6 M final)