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The principal compounds isolated were arctigenin and arctiin, both of which markedly suppressed IgE production by U266 cells in vitro, with arctigenin showing the strongest anti-IgE activity (IC 50 = 5.09 g/mL) [76]

Then, cells were harvested, washed twice with PBS, resuspended in Binding Buffer, and stained according to the manufacturers instructions using the Annexin V-FITC/PI Apoptosis Detection Kit (YEASEN, China)

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