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After purification, the protease is added to the eluted fractions to cleave the GST tag from the target protein

Even though they can be commercially acquired as a cell line, being readily characterized and accessible, one disadvantage lies in the fact that they may not express all the surface molecules as primary cells do, due to the immortalization process, making them a less reliable model of the in vivo behavior [88]

Several clinical trials have explored the potential of Medium Chain Triglycerides (MCTs) in enhancing cognitive function in AD and Mild Cognitive Impairment (MCI) patients, but the findings are still a topic of discussion

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