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The supernatant was collected from centrifugation at 15,000 g for 20 min at 4 C and protein was quantified with Bradford reagent
Nature (2013) 496:44555
You might get a temporary boost in speed, but without the right settings, you risk overstressing the system or having the performance drop the moment you stop refueling
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ELISA assays complement the HPLC analysis by measuring concentrations of melatonin and neurotrophic factors, utilizing kits from Sangon Biotechnology Company (Shanghai, China) following strict protocols to ensure reliability
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