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Before the ultrasound procedure, the mice were fasted for 8 h and intraperitoneally injected with 1.5% pentobarbital (45 mg/kg) 10 min prior to the procedure to anesthetize the animals

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This, in an effort to compensate for previous suppression of the release of DA, taking place during benzodiazepine administration, resulted in a sudden increase in DA concentrations [103]