Saline lacks the preservative necessary to prevent bacterial growth with repeated use
Motion correction was performed using Motioncor2 in Relion 4.0 45,46 , and CTF parameters were estimated with CTFFIND4 47
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In both methods, the addition of methanol was important to induce a complete phase transfer of DDA-GSH-AuNCs into the organic layer, in agreement with previous reports in which common solvents were used to facilitate phase transfer of nanoclusters and nanoparticles across biphasic liquid systems 32,46,47,48,49
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