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The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM

SCF/-TrCP promotes glycogen synthase kinase 3-dependent degradation of the Nrf2 transcription factor in a Keap1-independent manner

GABA is first converted back into glutamate by the mitochondrial enzyme GABA transaminase (GABA-T

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One of the most important conversations we have with patients involves scarring and long-term outcomes

Neurotransmitter binding to receptors Neurotransmitters function by binding to receptors on the cell membrane of the neuron releasing the neurotransmitter (i.e., presynaptic neuron) or to receptors on the cell membrane of the receiving cell (i.e., the postsynaptic neuron)