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To compare the expression of these genes in response to H 2 O 2 , we measured by qPCR the transcriptional level of each one in the pneumococcal R801strain (derivate of the R6 strain) [31] exposed at 20 mM H 2 O 2 for 30 min, and referred to gyrB as internal control [26]

The first question is relatively easy to answer

(2023) 5:e50822

Its a critical, non-negotiable element in the chain of quality that ensures your experiments are valid and your results are reproducible

doi: 10.1038/s41467-021-21998-4 32 ChatterjeeDLewisFJSuttonHJKaczmarskiJAGaoXCaiYet al

Step 3: Fix a Consistent Daily Time and Hold to It Glutathione depletion is continuous