sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml
Randomised trial of glutamine-enriched enteral nutrition on infectious morbidity in patients with multiple trauma
The association between maternal body mass index and child obesity: a systematic review and meta-analysis
Next steps Tips to help you get the most from a visit to your health care provider: Know the reason for your visit and what you want to happen
GLP-1 Drugs Are Rewriting the Rules of Food and Beverage July 02, 2026 DownloadsDownload Article When FTI Consulting began tracking the impact of GLP-1 weight-loss medications on consumer food demand in 2025, the central question was whether a pharmaceutical trend could become a structural economic force
EBioMedicine 2021, 65, 103249