For breeding, male VE-Cadherin-Cre ERT2 mice were crossed with female Igf1r f/f mice to generate VE-Cadherin-Cre ERT2 /Igf1r + / males, which were subsequently bred with Igf1r f/f females to establish the VE-Cadherin-Cre ERT2 /IGF1R homozygous floxed founder colony, following our previously described protocol [17, 45]
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Amino acids L-Arginine (A5131), L-Asparagine (A4159), L-Aspartic acid (A9256), L-Glutamic acid (G1251), L-Glycine (G8898), L-Lysine (L5626), L-Leucine (L8000), L-Isoleucine (I2752), L-Threonine (T8625), L-Tryptophan (T8941), and L-Valine (V0500) were obtained from Sigma
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Lancet 2015