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Vascular endothelial growth factor promotes physical wound repair and is anti-apoptotic in primary distal lung epithelial and A549 cells

Real-time PCR Total RNA was isolated from the cells using the Roche High Pure RNA isolation kit (Roche Life Science, 11828665001) and reverse transcription was performed using the iScript cDNA synthesis kit (Bio-Rad, 1708891) according to the manufacturers instructions

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Once reconstituted, the vial should be stored in the refrigerator at 2-8 degrees Celsius (35-46 degrees Fahrenheit)

If a CYP3A4 inducer is discontinued, consider hydrocodone bitartrate and acetaminophen tablets dosage reduction and evaluate patients at frequent intervals for signs of respiratory depression and sedation

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