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As the native GLP-1 has a short half-life, GLP-1 analogues have been produced to prolong effects and many of them could cross blood-brain barriers (BBB) such as Liraglutide (LG), lixisenatide, and exendin-4

Proteins were denatured in sample buffer and equal amounts were separated on SDS-PAGE and transferred to PVDF membrane (BioRad Laboratories, CA, USA)

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1 M), Ferrostatin-1 (Fer-1

IGF-1 LR3 (Insulin-like Growth Factor 1 Long Arg3) is a synthetic variant of human IGF-1 , modified to increase both its potency and half-life