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For sample cleanup, 1 L of Exo-SAP-IT (ThermoFisher) was added to 2.5 L of PCR product and incubated at 37 C for 15 min followed by a 15 min incubation at 80 C to inactivate the enzyme

MasoudiRSharafiMPourazadiLDadashpour DavachiNAsadzadehNEsmaeilkhanianSet al

Beyond biochemical activation, cellular interactions magnify thromboinflammation: activated platelets bind to NETs and to neutrophils via P-selectin/PSGL-1 and integrin pathways, forming plateletneutrophil aggregates that both promote further NET release and provide a nidus for coagulation complex assembly [66]

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