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Animal studies show no toxicity at therapeutic doses, but human data is insufficient for regulatory approval

J Leukoc Biol 102(2):343349 Fridlender ZG et al (2009) Polarization of tumor-associated neutrophil phenotype by TGF-beta: N1 versus N2 TAN

Briefly, HCT116 p53+/+ cells were collected, washed with PBS and diluted with cell lysis buffer (25 mM Tris-HCl (pH 7.5) and 10 mM MgCl 2 ) supplemented with complete protease inhibitor cocktail

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