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Criteria for necrosis included karyorrhexis (loss of nucleus) and/or degeneration of cytoplasm with either coagulative or liquefactive changes
Further from the collected genes, 31 of the genes, 25 genes were found to have a common match (HDAC1, SOD2, FOXO1, PPARGC1A, UBB, NTRK2, CREBBP, SIRT3, CDC34, PRKAA2, ATP5F1B, MAP4K4, PRKAA1, CDK1, PDE2A, ALOX5, IGF1R, RELA, EPHX2, MAP2K1, ADORA1, NTRK1, NTRK3, PRKDC, HIF1A) with the DisGeNET disease database
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TSB containing varying concentrations of NAC was added and plates incubated for an additional 48 h
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