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HEK293T cells (Cell Bank of Chinese Academy of Sciences, Shanghai, China) and human immortalized HSC cell line LX-2 (Procell Life Science & Technology, Wuhan, China) were cultured in DMEM (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin and streptomycin (Gibco, USA) at a humidified 37 C incubator with 5% CO 2

doi: 10.1371/journal.pone.0247060 15 BallatoriN.KranceS.MarchanR.HammondC

The hypothalamic-pituitary-adrenal axis and immune-mediated inflammation

For healing applications, most acute injuries show substantial improvement by week 8 to 10

These regional variations may correlate with cognitive and emotional dysfunctions observed in depression [406]

side-effect profile Considerations For example, amylin-based therapies can cause digestive effects, including nausea and vomiting, particularly during initiation or dose escalation