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Evidence that severe asthma can be divided pathologically into two inflammatory subtypes with distinct physiologic and clinical characteristics

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The samples were digested with DNase (Thermo Fisher Scientific), and 1 g of RNA was converted to first strand cDNA using Reversaid kit (Thermo Fisher Scientific) and random hexamers in 20 l final volume, according to the manufacturers protocol

Side effects and risks are possible

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Aspiration occurs when gastric contents enter the respiratory tract, potentially causing aspiration pneumonitis (chemical lung injury), aspiration pneumonia (infectious complication), or acute airway obstruction