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Alternative concentrations and when to use them The 3 mL standard works for most researchers

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The harvested cells were resuspended in buffer A (20 mM Tris-HCl, pH 8.0, 300 mM NaCl and 1 mM DTT) for GST-tagged proteins or in buffer B (20 mM Tris-HCl, pH 8.0, 300 mM NaCl, 5% glycerol and 10 mM imidazole) for His-tagged ShHTL7, and they were lysed by sonication