These cells were used between passages 6590 and cultured in RPMI media containing 11.1 mM glucose, 5% heat-inactivated FBS, 100 g/ml streptomycin, 100 units/ml penicillin sulphate, 100 units/ml neomycin, 50 M -mercaptoethanol, 10 mM HEPES and 1 mM sodium pyruvate
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