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The ratio is fixed

After that, cells expressing GIPR and GLP-1R were incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma), and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA, Abcam) at RT for 1 h

While freezing is an option for very long-term storage (many months to years), for most research timelines, refrigeration is perfectly sufficient.

5d) therefore suggests a less efficient interaction between E170X mutants and HEDS-derived GSSEtOH in accordance with the scaffold model and the hypothesis that GSSEtOH formation is catalysed by ScGrx7

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Intriguingly, an association between the prevalence of severe tetracycline-treated acne and increased risk of prostate cancer later in life has been observed [20]