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Methods Experimental animals The study was conducted using male TPH1 knockout mice and wild-type C57BL/6 mice (46 weeks, 2126 g) (Animal Feeding Center of Xi'an Jiaotong University Health Science Center)

Cells were dehydrated in a series of alcoholic solutions from 70 to 100%, followed by two immersions in propylene oxide for 20 min each, propylene oxide and araldite infiltration for 12 h, at room temperature on a shaking platform

A healthy gut is essential for reducing systemic inflammation and improving overall well-being

Do not use it if the solution appears cloudy or contains particles

The blend represents a middle ground between natural optimization and pharmaceutical intervention