Interestingly, at the age of 47 years P20 developed extensive surgical emphysema within the subcutaneous tissues of her face and computed tomography (CT) scans of the sinuses revealed a defect of the left nasal cartilages anteriorly allowing air to track into the soft tissues
Two cohorts of patients were identified
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Bacteriostatic Water (BAC Water) is the clear winner here
2448 h post-transfection, the transfected cells were identified and collected by fluorescence-activated cell sorting based on GFP fluorescence using a BD FACSAria II sorter (BD Biosciences, San Jose, CA) equipped with a GFP filter (530/30 nm)
Step 6: Label the vial with the reconstitution date and concentration