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doi: 10.3389/fnut.2025.1585922 Received 01 March 2025 Accepted 28 April 2025 Published 16 May 2025 Volume 12 - 2025 Edited by Mark Elisabeth Willems, University of Chichester, United Kingdom Reviewed by Luiz Royes, Federal University of Santa Maria, Brazil Alessandra DAmico, Sapienza University of Rome, Italy Lillian Morton, The University of Auckland, New Zealand Updates Copyright 2025 Wang and Yu

Real-time PCR was conducted for amplification with specific primers for the chondrogenic markers COL2A1 (Forward: 5-TTCAGCTATGGAGATGACAATC-3 and Reverse: 5-AGAGTCCTAGAGTGACTGAG-3), ACAN (Forward: 5-AGCCTGCGCTCCAATGACT-3 and Reverse: 5-TAATGGAACACGATGCCTTTCA-3), and SOX9 (Forward: 5-TTCCGCGACGTGGACAT-3 and Reverse: 5-TCAAACTCGTTGACATCGAAGGT-3)

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If you find that you cannot keep up with your fitness routine, it may indicate a deficiency