In humans, alpha, mu, pi and sigma isoenzymes contain a tyrosine in the G-site while the other classes a serine or a cysteine
Antibody binding was reported as the median fluorescence intensity (MFI) of IgG binding on the Luminex 100 (Luminex, Austin, TX, USA)
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It acted via non-competitive inhibition, binding to non-active sites and inducing structural rearrangements, with reduced -helical content and increased -sheet and random coil, thereby disrupting ACE function without active-site competition
doi: 10.1111/plb.13030 41 KnstlerA.KirlyL.KtayG.EnyediA