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Extended semen was packaged into 0.25 mL French mini-straws (IMV, France), equilibrated at 5 C for 4 h, frozen in liquid nitrogen (196 C) for at least one week, and thawed at 37 C for 30 s before evaluation

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Severe Heavy Metal Sensitivities: While not as clear-cut as Wilson's disease, a known, severe allergy or sensitivity to copper or other heavy metals is a major red flag